rabbit polyclonal anti-mouse gfap  (Merck KGaA)

Journal: eLife

Article Title: Switching of RNA splicing regulators in immature neuroblasts during adult neurogenesis

doi: 10.7554/eLife.87083

Figure Lengend Snippet:

Article Snippet: Antibody , Rabbit polyclonal anti-mouse Gfap , Merck Millipore , Cat#G9269, RRID: AB_477035 , 1:400.

Techniques: Recombinant, Red Blood Cell Lysis, Software, Staining, Immunolabeling, FACS

Journal: Inflammation Research

Article Title: Sigma-1 receptor targeting inhibits connexin 43 based intercellular communication in chronic neuropathic pain

doi: 10.1007/s00011-024-01926-0

Figure Lengend Snippet: (+)-MR200 mitigates reactive astrogliosis at spinal cord level. a Representative pictures of lumbar sections of spinal cord showing Gfap-positive cells on laminae I-IV; b Quantification of Gfap-positive cells per laminae area; c Representative pictures of lumbar sections of spinal cord showing Iba1-positive cells on laminae I-IV; d Quantification of Iba1-positive cells per laminae area. Data are shown as scattered dot plot and mean FC ± SD of n = 4 sections per group. CCI chronic constriction injury; FC fold change; SD standard deviation. ****p-value < 0.0001, *** p-value < 0.001, **p-value < 0.01 versus Sham vehicle, #### p-value < 0.0001, ### p-value < 0.001, ## p-value < 0.01, # p-value < 0.05 versus CCI vehicle. Scale bar 100 μm

Article Snippet: Then, slides were incubated overnight at + 4 °C with the following primary antibodies, diluted in 1% NGS or 1% NDkS and PBS-0,3% Triton:, anti-mouse monoclonal anti-NeuN antibody (Merck Millipore, Cat#MAB377, RRID: AB_2298772, 1:100), goat anti-AIF/Iba1 antibody (Novus Biologicals, Cat#NB100-1028, RRID: AB_521594, 1:100), rabbit polyclonal anti-mouse monoclonal anti-glial fibrillary acidic protein (Gfap) antibody (Santa Cruz Biotechnology, Cat#610,566, RRID: AB_397916, 1:100), rabbit polyclonal anti-Cleaved Caspase-3 (Asp175) antibody (Cell Signaling Technology Cat#9661, RRID: AB_2341188, 1:300), rabbit polyclonal anti-Cx43 antibody (Cell Signaling Technology Cat#3512, RRID: AB_2294590, 1:100).

Techniques: Standard Deviation

Journal: Inflammation Research

Article Title: Sigma-1 receptor targeting inhibits connexin 43 based intercellular communication in chronic neuropathic pain

doi: 10.1007/s00011-024-01926-0

Figure Lengend Snippet: (+)- MR200 rescues pro-apoptotic phenotype following CCI. a Representative confocal microscopy images of NeuN + Cl Casp3 + cells in ipsi-lateral dorsal horn. b Quantification of NeuN + Cl Casp3 + cells per mm 2 . c Representative confocal microscopy images of Gfap + Cl Casp3 + cells in ipsi-lateral dorsal horn. d Quantification of Gfap + Cl Casp3 + cells per mm 2 . e Representative confocal microscopy images of Iba1 + Cl Casp3 + cells in ipsi-lateral dorsal horn. f Quantification of Iba1 + Cl Casp3 + cells per mm 2 . Data are shown as scattered dot plot and mean FC ± SD of n = 4 sections per group. CCI chronic constriction injury; Cl Casp3, cleaved caspase 3; FC fold change; SD standard deviation. *** p-value < 0.001, **p-value < 0.01, *p-value < 0.05 versus Sham vehicle, ### p-value < 0.001, ## p-value < 0.01, # p-value < 0.05 versus CCI vehicle. Scale bar 10 μm

Article Snippet: Then, slides were incubated overnight at + 4 °C with the following primary antibodies, diluted in 1% NGS or 1% NDkS and PBS-0,3% Triton:, anti-mouse monoclonal anti-NeuN antibody (Merck Millipore, Cat#MAB377, RRID: AB_2298772, 1:100), goat anti-AIF/Iba1 antibody (Novus Biologicals, Cat#NB100-1028, RRID: AB_521594, 1:100), rabbit polyclonal anti-mouse monoclonal anti-glial fibrillary acidic protein (Gfap) antibody (Santa Cruz Biotechnology, Cat#610,566, RRID: AB_397916, 1:100), rabbit polyclonal anti-Cleaved Caspase-3 (Asp175) antibody (Cell Signaling Technology Cat#9661, RRID: AB_2341188, 1:300), rabbit polyclonal anti-Cx43 antibody (Cell Signaling Technology Cat#3512, RRID: AB_2294590, 1:100).

Techniques: Confocal Microscopy, Standard Deviation

Journal: Inflammation Research

Article Title: Sigma-1 receptor targeting inhibits connexin 43 based intercellular communication in chronic neuropathic pain

doi: 10.1007/s00011-024-01926-0

Figure Lengend Snippet: (+)- MR200 treatment influences Cx43-mediated cell-to-cell communication. a – e Confocal microscopy images of Gfap-positive astrocytes and Iba1-positive microglia expressing Cx43 in ipsilateral dorsal horn of Sham vehicle ( a ), CCI vehicle ( b ), CCI (+)-MR200 ( c ), CCI PRE084 ( d ), CCI PRE084 (+)-MR200 ( e ). a’–e’) Magnification and orthogonal projections showing Cx43 localization between astrocytes and microglia. f Quantification of Cx43 MFI over Gfap positive area and h) quantification of Cx43 MFI over Iba1 positive area. Data are shown as scattered dot plot and mean FC ± SD of n > 16 positive cells per group. g) Quantification of Gfap MFI over total area and i quantification of Iba1 MFI over total area. Data are shown as scattered dot plot and mean FC ± SD of n = 4 samples per group. CCI, chronic constriction injury; FC, fold change; MFI: mean fluorescence intensity; SD, standard deviation. ****p-value < 0.0001, **p-value < 0.01, *p-value < 0.05 versus Sham vehicle, #### p-value < 0.0001, ## p-value < 0.01, # p-value < 0.05 versus CCI vehicle. Scale bar 50 μm

Article Snippet: Then, slides were incubated overnight at + 4 °C with the following primary antibodies, diluted in 1% NGS or 1% NDkS and PBS-0,3% Triton:, anti-mouse monoclonal anti-NeuN antibody (Merck Millipore, Cat#MAB377, RRID: AB_2298772, 1:100), goat anti-AIF/Iba1 antibody (Novus Biologicals, Cat#NB100-1028, RRID: AB_521594, 1:100), rabbit polyclonal anti-mouse monoclonal anti-glial fibrillary acidic protein (Gfap) antibody (Santa Cruz Biotechnology, Cat#610,566, RRID: AB_397916, 1:100), rabbit polyclonal anti-Cleaved Caspase-3 (Asp175) antibody (Cell Signaling Technology Cat#9661, RRID: AB_2341188, 1:300), rabbit polyclonal anti-Cx43 antibody (Cell Signaling Technology Cat#3512, RRID: AB_2294590, 1:100).

Techniques: Confocal Microscopy, Expressing, Fluorescence, Standard Deviation